8IXV
Crystal structure of intracellular B30.2 domain of BTN3A in complex with 2Cl-HMBPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-27 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.774, 61.944, 75.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.210 - 1.720 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.101 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.770 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.048 | 0.218 |
| Number of reflections | 23485 | 1167 |
| <I/σ(I)> | 24.96 | |
| Completeness [%] | 98.9 | |
| Redundancy | 8.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | Calcium acetate hydrate, PEG8000,Sodium citrate |
