8IXU

Rat Transcobalamin in Complex with Cobalamin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	51.310, 54.530, 142.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.880 - 1.300
R-factor	0.1605
Rwork	0.160
R-free	0.17140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.896
Data reduction software	XDS (JAN 10 2022)
Data scaling software	XSCALE (JAN 10 2022)
Refinement software	PHENIX (1.20-4459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.290	48.290	1.330
High resolution limit [Å]	1.300	5.800	1.300
Rmerge	0.107	0.031	3.188
Rmeas	0.112	0.032	3.324
Number of reflections	99979	1262	7225
<I/σ(I)>	13.58	57.75	0.74
Completeness [%]	99.9
Redundancy	13.2704567959
CC(1/2)	1.000	1.000	0.277

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M NaNO3,20% PEG 3350, 0.1 M Bis-Tris propane pH 7.5