8IXU
Rat Transcobalamin in Complex with Cobalamin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-25 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.99 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.310, 54.530, 142.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.880 - 1.300 |
R-factor | 0.1605 |
Rwork | 0.160 |
R-free | 0.17140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.896 |
Data reduction software | XDS (JAN 10 2022) |
Data scaling software | XSCALE (JAN 10 2022) |
Refinement software | PHENIX (1.20-4459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.290 | 48.290 | 1.330 |
High resolution limit [Å] | 1.300 | 5.800 | 1.300 |
Rmerge | 0.107 | 0.031 | 3.188 |
Rmeas | 0.112 | 0.032 | 3.324 |
Number of reflections | 99979 | 1262 | 7225 |
<I/σ(I)> | 13.58 | 57.75 | 0.74 |
Completeness [%] | 99.9 | ||
Redundancy | 13.2704567959 | ||
CC(1/2) | 1.000 | 1.000 | 0.277 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M NaNO3,20% PEG 3350, 0.1 M Bis-Tris propane pH 7.5 |