8IXP

Apo structure of glycosyltransferase LmbT wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	97.536, 142.015, 158.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.770 - 2.450
R-factor	0.2045
Rwork	0.203
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	2.500
High resolution limit [Å]	2.450	2.450
Rmerge	0.155	1.080
Number of reflections	81741	4453
<I/σ(I)>	12.8	2.2
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.998	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.03M citrate acid, 0.07M BIS-tris propane pH7.6, 28% Polyethylene glycol 3,350