8IWL
A.baumannii Uncharacterized sugar kinase ydjH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2022-11-29 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.430, 84.300, 161.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.120 - 3.040 |
| R-factor | 0.2739 |
| Rwork | 0.273 |
| R-free | 0.29890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.779 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.120 | 3.149 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.143 | |
| Number of reflections | 12489 | 1194 |
| <I/σ(I)> | 12.41 | |
| Completeness [%] | 99.5 | |
| Redundancy | 13 | |
| CC(1/2) | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 12% PEG 3000, 0.1M Imidazole pH7.6, 0.2M Lithium sulfate |
