Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979021 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.388, 104.388, 145.334 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.640 - 2.860 |
| R-factor | 0.2045 |
| Rwork | 0.202 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.730 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.400 | 3.010 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Number of reflections | 21144 | 3146 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 96.9 | |
| Redundancy | 10.3 | |
| CC(1/2) | 0.998 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 31% PEG1500,0.1M Tris pH 8.4 |
