8IW2
Entamoeba histolytica Pyruvate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.801, 89.536, 37.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.937 - 1.750 |
| Rwork | 0.171 |
| R-free | 0.20170 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.899 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.937 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.115 | 0.956 |
| Rmeas | 0.109 | |
| Rpim | 0.047 | 0.485 |
| Number of reflections | 29332 | 1583 |
| <I/σ(I)> | 15.5 | 2 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 9.3 | 9 |
| CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 50mM MES buffer, pH 6.2, 0.2M LiCl2, 20% PEG 6000 |
