8IW0

Crystal structure of the KANK1/liprin-beta1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	48.151, 36.882, 93.011
Unit cell angles	90.00, 95.67, 90.00

Refinement procedure

Resolution	47.920 - 2.100
R-factor	0.2306
Rwork	0.227
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.541
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.920	2.170
High resolution limit [Å]	2.060	2.060
Rmerge	0.154	0.829
Rmeas	0.169	0.914
Number of reflections	20404	2971
<I/σ(I)>	8.2	2.5
Completeness [%]	99.2	99.2
Redundancy	5.8	5.6
CC(1/2)	0.994	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2% v/v TacsimateTM pH 7.0, 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 3,350