8IVK
Cryogenic temperature crystal structure of Escherichia coli CyaY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PhotonJet-R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-16 |
Detector | RIGAKU HyPix-3000 |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 44.456, 44.456, 98.425 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.230 - 1.500 |
R-factor | 0.1804 |
Rwork | 0.176 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.482 |
Data reduction software | CrysalisPro (1.171.42.35a) |
Data scaling software | CrysalisPro (1.171.42.35a) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.230 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 18719 | 1821 |
<I/σ(I)> | 14.11 | |
Completeness [%] | 99.9 | |
Redundancy | 6 | |
CC(1/2) | 0.998 | 0.582 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 0.1 M Citric acid, 4.0 M Sodium chloride (pH 4.0) |