8IVD
COMPLEX STRUCTURE OF CD93-IGFBP7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 131.512, 208.895, 77.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 3.240 |
| R-factor | 0.2464 |
| Rwork | 0.244 |
| R-free | 0.30180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.464 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 3.500 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rmerge | 0.200 | 2.140 |
| Rmeas | 0.200 | |
| Rpim | 0.090 | 1.000 |
| Number of reflections | 17340 | 3495 |
| <I/σ(I)> | 9.4 | 1.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12 | 10.4 |
| CC(1/2) | 0.996 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M imidazole, 20% w/v PEG monomethyl ether 2000, and 0.2 M ammonium citrate tribasic pH 7.0 |
