8IVD

COMPLEX STRUCTURE OF CD93-IGFBP7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9790
Spacegroup name	C 2 2 21
Unit cell lengths	131.512, 208.895, 77.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 3.240
R-factor	0.2464
Rwork	0.244
R-free	0.30180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.464
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	3.500
High resolution limit [Å]	3.240	3.240
Rmerge	0.200	2.140
Rmeas	0.200
Rpim	0.090	1.000
Number of reflections	17340	3495
<I/σ(I)>	9.4	1.3
Completeness [%]	99.9	99.9
Redundancy	12	10.4
CC(1/2)	0.996	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M imidazole, 20% w/v PEG monomethyl ether 2000, and 0.2 M ammonium citrate tribasic pH 7.0