8IRW
Structure of nanobody Nb9 against parathion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.662, 50.397, 56.123 |
| Unit cell angles | 90.00, 96.39, 90.00 |
Refinement procedure
| Resolution | 37.390 - 1.550 |
| R-factor | 0.1945 |
| Rwork | 0.193 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.887 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.390 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.066 | 0.637 |
| Number of reflections | 33692 | 1651 |
| <I/σ(I)> | 19.4 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6 |
| CC(1/2) | 0.999 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293.15 | 7M sodium nitrate |
