8IQX
ferritin mutant-P156H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 229.157, 229.157, 229.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.640 - 2.500 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.981 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.18.2_3874) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.640 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.161 | |
| Number of reflections | 1269561 | 68784 |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 18.5 | |
| CC(1/2) | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM imidazole, 15%(v/v) reagent alcohol, 200 mM magnesium chloride, 2.0 mM nickel chloride |
