8IQA

Crystal structure of Pyruvic Oxime Dioxygenase (POD) from Alcaligenes faecalis deleted N-terminal 18 residues

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	49.220, 135.045, 138.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.680 - 1.551
R-factor	0.1918
Rwork	0.191
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.930
Phasing software	MOLREP (11.9.02)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.530	1.680
High resolution limit [Å]	1.550	1.550
Rmerge	0.101	0.953
Number of reflections	95150	4756
<I/σ(I)>	10.7
Completeness [%]	94.0
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	20% (w/v) PEG 3350, 8%(v/v) tacsimate