8IPW
The sturecture of Legionella effector protein MavL with ADPR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.790, 108.320, 129.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.240 - 2.350 |
| R-factor | 0.2126 |
| Rwork | 0.209 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6omi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.4 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.110 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.380 |
| Number of reflections | 30117 | 1970 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.998 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 289.15 | 0.25 M potassium citrate tribasic monohydrate,18%PEG3350 |
