8IPL
The structure of human mitochondrial methyltransferase METTL15 with RBFA and SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.167, 70.978, 75.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.477 - 2.200 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (3.3.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.480 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.111 | 0.507 |
| Number of reflections | 16587 | 789 |
| <I/σ(I)> | 16.5 | 6.2 |
| Completeness [%] | 92.6 | |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 17% w/v PEG 4000, 0.05M Potassium chloride, 0.1M Lithium chloride, 0.012M Spermine tetrahydrochloride, 0.05M MES PH6.5 |
