8IPK
The structure of human mitochondrial methyltransferase METTL15 with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.506, 70.506, 134.674 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.245 - 1.900 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.22070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.784 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (3.3.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.250 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.126 | 1.003 |
| Number of reflections | 31270 | 1647 |
| <I/σ(I)> | 23.2 | 2.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 42% PEG 600, 0.2 M imidazole malate, pH 5.5 |
