8IPI
The apo structure of human mitochondrial methyltransferase METTL15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.607, 70.607, 136.063 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.336 - 2.100 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.793 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (3.3.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.340 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.148 | 0.800 |
| Number of reflections | 23694 | 1134 |
| <I/σ(I)> | 15.8 | 2.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 17.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 10% PEG MME 2000, 0.2M Ammonium sulfate, 0.1 M sodium acetate (pH 5.5) |
