8IOZ
Crystal structure of transaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17UM |
| Synchrotron site | SSRF |
| Beamline | BL17UM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 71.443, 71.443, 499.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.080 - 2.330 |
| R-factor | 0.20815 |
| Rwork | 0.205 |
| R-free | 0.26533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 34208 | 6105 |
| <I/σ(I)> | 22.2 | 1.87 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 40.7 | 38.7 |
| CC(1/2) | 0.996 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 35% Tacsimate pH7.0,0.1 M Bis-Tris propane pH 6.5 |
