8IOO
Crystal structure of Deinococcus radiodurans RecJ-like protein in complex with Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2022-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.897, 124.842, 80.917 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 42.040 - 2.000 |
| R-factor | 0.2284 |
| Rwork | 0.228 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.245 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.040 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.358 |
| Number of reflections | 106336 | 7618 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.5 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.4 M MgSO4, 0.08 M MES (pH 6.5), 15% (v/v) glycerol |
