8INV

Crystal structure of UGT74AN3-UDP-BUF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	203
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	45.682, 52.281, 200.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 1.850
R-factor	0.1854
Rwork	0.184
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.760	1.890
High resolution limit [Å]	1.850	1.850
Number of reflections	42800	2584
<I/σ(I)>	10.3
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.989	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG2000MME, 0.1M Hepes (7.5), 0.2M potassium nitro