8INO

Crystal structure of UGT74AN3 in complex UDP and PER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	203
Detector technology	PIXEL
Collection date	2021-07-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	45.608, 52.200, 200.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 2.300
R-factor	0.1863
Rwork	0.184
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.830	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	22111	2152
<I/σ(I)>	14.7
Completeness [%]	99.8
Redundancy	25.7
CC(1/2)	0.995	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG2000MME, 0.1M Hepes (7.5), 0.2M potassium nitro