8INH
ZjOGT3, flavonoid 7,4'-di-O-glycosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.730, 84.810, 103.630 |
| Unit cell angles | 90.00, 93.33, 90.00 |
Refinement procedure
| Resolution | 51.730 - 2.500 |
| R-factor | 0.2415 |
| Rwork | 0.240 |
| R-free | 0.27610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.481 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.730 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 35988 | 35988 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 97.5 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.984 | 0.984 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M sodium acetate trihydrate, 26% w/v PEG 4000, 10 mM Tris-HCl, pH 8.0 |
