8IN9
The structure of the GfsA KSQ-AT didomain in complex with the GfsA ACP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 102.212, 102.212, 408.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.330 - 3.400 |
| R-factor | 0.2674 |
| Rwork | 0.265 |
| R-free | 0.31070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.725 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.063 | 1.334 |
| Number of reflections | 15447 | 3126 |
| <I/σ(I)> | 19.4 | 1.9 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 10.3 | 10.8 |
| CC(1/2) | 1.000 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | ammonium sulfate, Tris-HCl, Pentaerythritol ethoxylate |
