8IN4

Eisenia hydrolysis-enhancing protein from Aplysia kurodai

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.576, 65.553, 67.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.020 - 1.400
R-factor	0.1666
Rwork	0.166
R-free	0.18410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.839
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.020	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	35930	3486
<I/σ(I)>	14.34
Completeness [%]	99.3
Redundancy	14.34
CC(1/2)	0.999	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.0 M sodium acetate, 0.1 M imidazole (pH 6.5)