8IN3

Eisenia hydrolysis-enhancing protein from Aplysia kurodai

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	42.180, 65.330, 66.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.620 - 1.150
R-factor	0.1823
Rwork	0.182
R-free	0.18920
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.195
High resolution limit [Å]	1.150	1.150
Number of reflections	60994	4888
<I/σ(I)>	19.28
Completeness [%]	93.4
Redundancy	6.6
CC(1/2)	0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.0 M sodium acetate, 0.1 M imidazole (pH 6.5)