8IMW
Crystal structure of response regulator PmrA receiver domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.109, 39.057, 92.188 |
| Unit cell angles | 90.00, 95.86, 90.00 |
Refinement procedure
| Resolution | 31.941 - 1.600 |
| R-factor | 0.1909 |
| Rwork | 0.188 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (v1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.941 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 30050 | 2921 |
| <I/σ(I)> | 18.56 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.999 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium malonate pH 6.0, 12% w/v Polyethylene glycol 3,350 |
