8ILZ

Crystal structure of the RRM domain of human SETD1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97915
Spacegroup name	P 65
Unit cell lengths	70.575, 70.575, 37.573
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.290 - 1.770
R-factor	0.1786
Rwork	0.177
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.869
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.290	1.810
High resolution limit [Å]	1.770	1.770
Rmerge	0.053	0.461
Number of reflections	10501	10501
<I/σ(I)>	35.29
Completeness [%]	99.5
Redundancy	18.8
CC(1/2)	1.000	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 9.0, 27% PEG 4000, 9% glycerol