8ILY

Crystal structure of the RRM domain of human SETD1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	40.542, 31.675, 65.579
Unit cell angles	90.00, 94.60, 90.00

Refinement procedure

Resolution	33.200 - 1.700
R-factor	0.184
Rwork	0.182
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.410	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.053
Number of reflections	18532	18532
<I/σ(I)>	21.32
Completeness [%]	99.5
Redundancy	6.2
CC(1/2)	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	4M Sodium formate