8ILX
mCherry-LaM3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-26 |
| Detector | DECTRIS EIGER2 X 4M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.867, 104.831, 68.508 |
| Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
| Resolution | 66.800 - 3.290 |
| R-factor | 0.2661 |
| Rwork | 0.264 |
| R-free | 0.31190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.771 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.970 | 3.380 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.447 | 2.359 |
| Rmeas | 0.491 | 2.619 |
| Rpim | 0.199 | 1.120 |
| Total number of observations | 93219 | 5864 |
| Number of reflections | 14166 | 1033 |
| <I/σ(I)> | 4.7 | 1.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.6 | 5.7 |
| CC(1/2) | 0.818 | 0.346 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.2 M sodium sulfate, 0.1 M Bis-Tris propane (pH 7.5), 20% (w/v) PEG 3350 |
