8ILW

Transcription factor LMX1a homeobox domain in complex with Pitx3 promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	46.693, 51.486, 102.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.588 - 2.712
R-factor	0.2512
Rwork	0.248
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.310
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (3.3.22)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.590	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.118	0.660
Number of reflections	7085	536
<I/σ(I)>	29.1	2
Completeness [%]	99.3
Redundancy	9.7
CC(1/2)	0.940	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20% PEG 3350, 0.2 mM sodium acetate