8ILL

Crystal structure of a highly photostable and bright green fluorescent protein at pH5.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-12
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	133.999, 133.999, 59.023
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.000 - 2.200
R-factor	0.1568
Rwork	0.154
R-free	0.18950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.670
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.050	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.171	0.463
Rmeas	0.179	0.508
Rpim	0.055	0.204
Number of reflections	30891	1479
<I/σ(I)>	11.1	2.6
Completeness [%]	99.8	96.48
Redundancy	10.6	6.07
CC(1/2)	0.994	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	20 %(w/v) PEG 4000, 20%(v/v) 2-propanol, 0.1 M Sodium Citrate (pH 5.6)