8ILK

Crystal structure of a highly photostable and bright green fluorescent protein at pH8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-12
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	53.876, 44.851, 93.788
Unit cell angles	90.00, 103.44, 90.00

Refinement procedure

Resolution	91.220 - 1.560
R-factor	0.1872
Rwork	0.186
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.594
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.220	1.590
High resolution limit [Å]	1.560	1.560
Rmerge	0.070	0.425
Rmeas	0.077	0.480
Rpim	0.032	0.218
Number of reflections	62074	2856
<I/σ(I)>	9.2	4.6
Completeness [%]	99.5
Redundancy	5.6
CC(1/2)	0.999	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	25 % PEG 3350, 0.2 M MgCl2, 0.1 M Tris-HCl (pH8.5)