8ILI
The crystal structure of dG(Se-Rp)-DNA:Pol X product binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-05-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.420, 87.344, 87.480 |
| Unit cell angles | 90.00, 91.16, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.21657 |
| Rwork | 0.215 |
| R-free | 0.24293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.060 | 0.037 | 0.264 |
| Total number of observations | 346977 | ||
| Number of reflections | 69608 | 7272 | 6191 |
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 97.1 | 99.5 | 87.4 |
| Redundancy | 5 | 6.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | ammonium sulfate (0.2 M), Bis-Tris pH 6.5 (0.1 M), 20% PEG3350 |
