8ILI

The crystal structure of dG(Se-Rp)-DNA:Pol X product binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-05-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	60.420, 87.344, 87.480
Unit cell angles	90.00, 91.16, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.21657
Rwork	0.215
R-free	0.24293
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.226
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.060	0.037	0.264
Total number of observations	346977
Number of reflections	69608	7272	6191
<I/σ(I)>	9.6
Completeness [%]	97.1	99.5	87.4
Redundancy	5	6.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	ammonium sulfate (0.2 M), Bis-Tris pH 6.5 (0.1 M), 20% PEG3350