8ILE
The crystal structure of dGTPalphaSe-Rp:DNApre-II:Pol X substrate ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.481, 77.382, 111.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 3.000 |
| R-factor | 0.22365 |
| Rwork | 0.220 |
| R-free | 0.28371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.416 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.117 | 0.098 | 1.225 |
| Total number of observations | 138097 | ||
| Number of reflections | 10889 | 1180 | 1063 |
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 99.9 | 99.7 | 100 |
| Redundancy | 12.7 | 11.6 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M L-proline, HEPES pH 8.6 (0.1 M),10% PEG 3350 |
