8ILA
Crystal structure of LmbT from Streptomyces lincolnensis NRRL ISP-5355 in complex with substrates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 122.382, 137.739, 146.101 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.870 - 2.790 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.184 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.220 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.091 | |
| Number of reflections | 62181 | 9020 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.1 | |
| CC(1/2) | 0.998 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8% PEG 20000, 0.05 M Tris pH 8.5, 1.2 M Sodium formate |
