8IL8
Crystal structure of Pyruvic Oxime Dioxygenase (POD) from Alcaligenes faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2022-06-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.002, 107.552, 87.794 |
| Unit cell angles | 90.00, 128.07, 90.00 |
Refinement procedure
| Resolution | 76.440 - 1.769 |
| R-factor | 0.1836 |
| Rwork | 0.182 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.920 |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.440 | 1.900 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.056 | 0.565 |
| Number of reflections | 55281 | 2766 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 93.5 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 36% (v/v) PEG 400, 0.2M calcium acetate, 0.1M HEPES |
