8IL5

Crystal structure of alcohol oxidase PcAOX(M59V/Q60P/R61N)(Phanerochaete chrysosporium)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9769
Spacegroup name	P 4 21 2
Unit cell lengths	161.260, 161.260, 113.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.182 - 2.500
R-factor	0.168335889111
Rwork	0.166
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.309
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.010	2.520
High resolution limit [Å]	2.460	2.460
Number of reflections	54647	3996
<I/σ(I)>	14.9
Completeness [%]	99.9
Redundancy	51
CC(1/2)	0.998	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	1.6 M ammonium sulfate, 0.1M MES pH 6.5, 10% (v/v) 1,4-Dioxane