8IL4
Crystal structure of alcohol oxidase ParAOX(M59V/Q60P/R61N/F101S/N602H)(Polyporus arcularius)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2022-10-02 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.706, 187.433, 118.532 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 45.567 - 3.355 |
| R-factor | 0.196133617571 |
| Rwork | 0.194 |
| R-free | 0.24318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.326 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.540 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 42191 | 5598 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 94.1 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.977 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 200 mM Sodium chloride, 100 mM HEPES pH 7.5, 30% PEG 400 |
