8IJS
anti-VEGF nanobody mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.915, 53.205, 65.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.170 - 1.752 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5imk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.170 | 1.814 |
| High resolution limit [Å] | 1.752 | 1.752 |
| Number of reflections | 9121 | 781 |
| <I/σ(I)> | 41.85 | |
| Completeness [%] | 98.5 | |
| Redundancy | 11.8 | |
| CC(1/2) | 0.997 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294.15 | PEG 8000, Zn(OAc)2, MES |
