8IIT
Complex form of MsmUdgX H109S/R184A double mutant and uracil- obtained from uracil DNA (ttUtt) post its cleavage by MsmUdgX H109S/R184A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-04-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.440, 51.420, 54.940 |
| Unit cell angles | 90.00, 104.84, 90.00 |
Refinement procedure
| Resolution | 23.590 - 1.610 |
| R-factor | 0.1571 |
| Rwork | 0.156 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.685 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.550 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | 0.276 |
| Rmeas | 0.061 | 0.360 |
| Rpim | 0.038 | 0.229 |
| Number of reflections | 25385 | 908 |
| <I/σ(I)> | 18.5 | 6 |
| Completeness [%] | 98.1 | 71.8 |
| Redundancy | 4.4 | 3.8 |
| CC(1/2) | 0.995 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
