8IIS
MsmUdgX H109S/R184A double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-04-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.360, 51.650, 54.580 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 23.480 - 1.610 |
| R-factor | 0.1538 |
| Rwork | 0.153 |
| R-free | 0.17030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.746 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.110 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.059 |
| Rmeas | 0.051 | 0.076 |
| Rpim | 0.031 | 0.046 |
| Number of reflections | 24280 | 831 |
| <I/σ(I)> | 28.2 | 16.8 |
| Completeness [%] | 94.3 | 66.2 |
| Redundancy | 4.9 | 4.2 |
| CC(1/2) | 0.996 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
