8IIP
Complex form of MsmUdgX H109Q mutant and uracil- obtained from uracil DNA (ttUtt) post its cleavage by MsmUdgX H109Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-04-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.560, 51.340, 54.880 |
| Unit cell angles | 90.00, 104.67, 90.00 |
Refinement procedure
| Resolution | 23.580 - 1.630 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.925 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.130 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.068 | 0.520 |
| Rmeas | 0.088 | 0.672 |
| Rpim | 0.055 | 0.420 |
| Number of reflections | 24686 | 1270 |
| <I/σ(I)> | 12.7 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.7 | 4.5 |
| CC(1/2) | 0.996 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
