8IIN
Complex form of MsmUdgX H109K mutant and uracil- obtained from uracil DNA (ttUtt) post its cleavage by MsmUdgX H109K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-04-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.460, 51.540, 54.860 |
| Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
| Resolution | 23.590 - 1.610 |
| R-factor | 0.1625 |
| Rwork | 0.162 |
| R-free | 0.18340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.120 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.568 |
| Rmeas | 0.082 | 0.729 |
| Rpim | 0.050 | 0.453 |
| Number of reflections | 24583 | 845 |
| <I/σ(I)> | 13.7 | 2.9 |
| Completeness [%] | 95.0 | 66.6 |
| Redundancy | 4.9 | 4.4 |
| CC(1/2) | 0.997 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
