8IIL
Complex form of MsmUdgX H109G mutant and uracil- obtained from uracil DNA (ttUtt) post its cleavage by MsmUdgX H109G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-07-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.780, 50.830, 55.420 |
| Unit cell angles | 90.00, 105.08, 90.00 |
Refinement procedure
| Resolution | 36.850 - 2.300 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.999 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.510 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.123 | 0.592 |
| Rmeas | 0.160 | 0.771 |
| Rpim | 0.100 | 0.487 |
| Number of reflections | 8891 | 828 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.8 | |
| CC(1/2) | 0.991 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
