8IH4
Crystal Structure of Intracellular B30.2 Domain of BTN2A2 Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-25 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.673, 59.843, 127.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.940 - 2.120 |
| R-factor | 0.2071 |
| Rwork | 0.204 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zxk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.545 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.940 | 2.150 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.106 | 0.488 |
| Number of reflections | 24957 | 1012 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M sodium citrate tribasic dihydrate, 20% w/v polyethylene glycol 3350 |
