8IH1
Room temperature structure of GH11 from Thermoanaerobacterium saccharolyticum by serial crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 298.15 |
| Detector technology | PIXEL |
| Collection date | 2019-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.450, 74.450, 167.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.050 - 1.750 |
| R-factor | 0.23559 |
| Rwork | 0.233 |
| R-free | 0.29296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.628 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 172.400 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 49353 | 4815 |
| <I/σ(I)> | 12.73 | |
| Completeness [%] | 100.0 | |
| Redundancy | 775.8 | |
| CC(1/2) | 0.992 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 4.0 M ammonium acetate |
