8IGT

Crystal Structure of Intracellular B30.2 Domain of BTN2A1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	73.957, 78.889, 41.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.670 - 1.560
R-factor	0.1558
Rwork	0.155
R-free	0.17320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zxk
RMSD bond length	0.014
RMSD bond angle	1.824
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.590
High resolution limit [Å]	1.560	4.230	1.560
Rmerge	0.095	0.052	0.471
Rmeas	0.099	0.054	0.495
Rpim	0.027	0.015	0.151
Total number of observations	454210
Number of reflections	35687	1955	1647
<I/σ(I)>	4.7
Completeness [%]	99.6	99.4	93.5
Redundancy	12.7	11.9	10.1
CC(1/2)		0.998	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.4 M potassium sodium tartrate