8IGL
Crystal structure of a major fragment of the ASFV inner capsid protein p150
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.440, 65.600, 115.010 |
| Unit cell angles | 99.21, 92.70, 90.16 |
Refinement procedure
| Resolution | 32.540 - 2.400 |
| R-factor | 0.2406 |
| Rwork | 0.238 |
| R-free | 0.28340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.888 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (Version 1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.058 | 0.212 |
| Number of reflections | 66557 | 4462 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 95.2 | 94.3 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 12%PEG 3350, 0.1M CHES at pH 9.0 |
