8IEV

Crystal structure of the DUF2891 family protein CJ0554 from Campylobacter jejuni in space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	306.168, 62.423, 134.879
Unit cell angles	90.00, 112.45, 90.00

Refinement procedure

Resolution	29.670 - 2.080
R-factor	0.1798
Rwork	0.178
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.866
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.120
High resolution limit [Å]	2.080	2.080
Number of reflections	141798	7047
<I/σ(I)>	16.7	2.5
Completeness [%]	99.6	99.7
Redundancy	3.3	3.2
CC(1/2)	0.994	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	sodium citrate, PEG3350