8IEU

Crystal structure of the DUF2891 family protein CJ0554 from Campylobacter jejuni in space group P41212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	99.465, 99.465, 211.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.1823
Rwork	0.181
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.793
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	47752	2356
<I/σ(I)>	11.3	2.7
Completeness [%]	99.5	99.8
Redundancy	4.7	4.6
CC(1/2)	0.982	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	sodium acetate, lithium sulfate