8IDV
Crystal structure of Fic protein from Mycoplasma mycoides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.600, 120.600, 165.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.190 - 3.500 |
| R-factor | 0.22197 |
| Rwork | 0.214 |
| R-free | 0.29713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.581 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.710 |
| High resolution limit [Å] | 3.499 | 3.500 |
| Rmerge | 0.153 | 0.921 |
| Rmeas | 0.168 | 0.966 |
| Number of reflections | 18055 | 2873 |
| <I/σ(I)> | 10.56 | 1.62 |
| Completeness [%] | 95.6 | 88.7 |
| Redundancy | 18.5 | 11.6 |
| CC(1/2) | 0.998 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES, 22% PEG400 |
